Protein Labeling Reagents
Abcam Metho x y- x 04, amyloid beta fluorescent marker, 5MG
MW 344.4 Da, Purity >98%. Blood-brain barrier permeable amyloid β fluorescent marker. Blue fluorophore. Derivative of Congo Red (ab145645) and Chrysamine G. Selectively binds fibrillar β-sheet deposits with high affinity (Ki = 26.8 nM). Detects plaques, tangles and cerebrovascular amyloid in vivo. .
The product is subject to the following: Abcam Restricted Use Statement
AdipoGen 5-TAMRA N-succinimidyl ester
Chemical. CAS 150810-68-7. Formula C29H25N3O7. MW 527.52. Synthetic. Amine-reactive form of 5-carboxytetramethylrhodamine single isomer. Conjugates yield bright, pH-insensitive orange-red fluorescence Ex/Em ~546/579nm with good photostability.
Cayman Chemical BIotIn hydrazIde 100mg
A biotinyl derivative used to label surface functional groups, antibodies, lectins, sugars, nucleic acids, or molecules with free carboxylic or keto groups; probe for determining protein carbonylation
AdipoGen 5-TAMRA
Chemical. CAS 91809-66-4. Formula C25H22N2O5. MW 430.45. Synthetic. Free acid form of 5-carboxytetramethylrhodamine single isomer. Widely used fluorophore for preparing bioconjugates, especially fluorescent antibody and avidin derivatives used in immunochemistry. Used as a dye for oligonucleotide labeling and automated DNA sequencing applications.
Medchemexpress LLC Sulfo-nhs-lc-biotin sodium | 191671-46-2 | 98.0% | 50 MG
Sulfo-NHS-LC-Biotin sodium is an amine-reactive ester derivative of biotin that cannot cross the intact blood-retinal barrier. It is utilized to assess the vascular permeability of both the brain and retinal vasculature. The product is for research use only.
- Reacts with amines to form a stable bond.
- Does not pass the blood-retinal barrier.
- Can be used to evaluate permeability in the brain and retinal blood vessels.
- Available in solid form.
- White to yellow in color.
- Stable at room temperature for shipping.
- Store at 4°C, sealed, and away from moisture.
Cayman Chemical PhosphIn-bIotIn 5mg
A labeling reagent that selectively reacts with azido groups on modified proteins through the Staudinger ligation reaction; can be used in common avidin-based biochemical techniques in whole cells or by blotting experiments following SDS-PAGE; has been used successfully in conjunction with Daz-1 or Daz-2 to label and detect sulfenic acid sites in proteins
Vector Laboratories Wheat Germ Agglutinin (WGA) Fluorescein-labeled 5 mg
Wheat germ agglutinin (WGA) contains a group of closely related isolectins that bind N-acetylglucosamine (preferentially the dimers and trimers of this sugar) and oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA also interacts with some glycoproteins via sialic acid residues (see succinylated WGA). This fluorescein-labeled WGA features a ratio of fluorophores to lectin protein that provides optimal staining (excitation 495 nm, emission 515 nm). Supplied as a solution essentially free of unconjugated fluorophores, it is preserved with sodium azide. The recommended inhibiting/eluting sugar is chitin hydrolysate or 500 mM N-acetylglucosamine with salt and/or acid elution.
Vector Laboratories Concanavalin A (Con A) Fluorescein 25 mg
Concanavalin A (ConA) recognizes α-linked mannose residues present as part of a "core oligosaccharide" in many serum and membrane-bound glycoproteins. At neutral and alkaline pH, Con A exists as a tetramer, but below pH 5.6, it dissociates into dimers of 52 kDa. Derivatizations can produce stable forms with dimeric structures. Con A requires calcium or manganese ions at each binding site, so avoid using buffers that bind calcium (such as phosphate) to dilute Con A since a gradual loss in activity may occur. This fluorescein-labeled Con A features a ratio of fluorophores to lectin protein that provides optimal staining (excitation 495 nm, emission 515 nm). Supplied as a solution essentially free of unconjugated fluorophores, it is preserved with sodium azide. The recommended inhibiting/eluting sugar is a mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside.
Biotium CD55 (DAF)(143-30), CF568 conjugate, 0.1mg/mL
Recognizes a single chain glycoprotein of 70 kDa, identified as CD55 (also known as decay accelerating factor, DAF). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. The MAb 143-30 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405
Biotium Complement C4d(C4D205), CF488A conjugate, 0.1mg/mL
This MAb is specific to Complement 4d (C4d) and it reacts with the secreted as well as cell-bound C4d.C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, a
Biotium CD11a(DF1524), CF488A conjugate, 0.1mg/mL
Recognizes a protein of 180 kDa, identified as CD11a (Leucocyte Workshop IV; Code 1524). CD11a complex with the 2 subunit of the integrin family, CD18, to form the cell surface heterodimer, LFA-1 or CD11a /C18 (aLbL). LFA-1 is expressed on all leukocytes including lymphocytes, monocytes, and granulocytes. It is involved in leukocyte adhesion to its ligands including intercellular adhesion molecule-1 (ICAM-1 or CD54), ICAM-2 (CD102), ICAM-3 (CD50) and Telencephalin (TLN) and play a role in most immune/inflammatory responses. This MAb potently blocks LFA-1 dependent homotypic cell aggregation.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye co
Biotium CD28(C28/77), CF488A conjugate, 0.1mg/mL
Recognizes a glycoprotein of 44-88 kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.